Use of bacteriophage for biological control of Salmonella Enteritidis infection in chicken

作者：Lim Tae Hyun; Kim Myung Seob; Lee Dong Hun; Lee Yu Na; Park Jae Keun; Youn Ha Na; Lee Hyun Jeong; Yang Si Yong; Cho Young Wook; Lee Joong Bok; Park Seung Yong; Choi In Soo; Song Chang Seon^*

来源：Research in Veterinary Science, 2012, 93(3): 1173-1178.

DOI：10.1016/j.rvsc.2012.06.004

• Salmonella Enteritidis
• Bacteriophage
• Bactericidal activity
• Feed additives
• Colonization
• Environmental contamination

Bacteriophage Phi CJ07 with broad host ranges for Salmonella strains isolated from sewage effluent were used to reduce Salmonella Enteritidis (SE) infection in chickens. One-day-old chicks challenged with 5 x 10(7) colony-forming units/bird of SE were cohabitated with contact chicks and treated with three concentrations (10(5), 10(7) and 10(9) plaque forming units (PFU)/g) of bacteriophage prepared as a feed additive for 21 days after challenge. Salmonella in the intestine was quantified and environmental contamination level was examined at 1.2 and 3 weeks after challenge. All treatments reduced intestinal SE colonization in challenged and contact chickens and reduced the environmental contamination level, but the reductions produced by 10(7) and 10(9) PFU/g of bacteriophage were significant (P %26lt; 0.05) as compared with untreated controls. In addition, seven out of 10 (70%) contact chickens treated with 10(9) PFU/g of bacteriophage had no detectable intestinal Salmonella at 3 weeks after treatment, suggesting that bacteriophage therapy significantly prevented the horizontal transmission of SE. These results provide important insights into preventive and control strategies against SE infection in poultry and indicate that the use of bacteriophage could reduce the incidence of Salmonella food poisoning.

• 出版日期2012-12