科研之友
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摘要
Casticin, chrysoplenol-D, p-hydroxy benzoic acid, and p-methoxy benzoic acid are representative flavonoids and benzoic acid derivatives in Vitex trifolia, a well-known traditional Chinese, as well as ayurvedic medicine, with a wide range of biological activities. In the present paper, the aforementioned four markers were isolated and simultaneously quantified by TLC densitometric method. In the present study, a novel stability-indicating high-performance thin-layer chromatography (HPTLC) method for quantitative determination of the aforementioned four markers has been developed and validated on silica using solvent dichloromethane-methanol-formic acid (9.6:0.4:0.06 v/v/v) in the absorbance mode at 254 nm. The method showed a good linear relationship (r(2) = 0.999) in the concentration range 100-2400 ng per band. It was found to be linear, accurate, precise, specific, robust, and stability-indicating and can be applied for quality control and standardization of other multi-component formulations based on Vitex trifolia.
- 出版日期2011
- 单位河北医科大学
