ATL54, a ubiquitin ligase gene related to secondary cell wall formation, is transcriptionally regulated by MYB46

作者：Noda Soichiro^*; Yamaguchi Masatoshi; Tsurumaki Yuta; Takahashi Yoshinori; Nishikubo Nobuyuki; Hattori Takefumi; Demura Taku; Suzuki Shiro; Umezawa Toshiaki

来源：Plant Biotechnology, 2013, 30(5): 503-U120.

DOI：10.5511/plantbiotechnology.13.0905b

• Arabidopsis thaliana
• E3 ubiquitin ligase
• secondary cell wall formation
• transcription factor

We previously characterized Arabidopsis Toxicos en Levadura54 (ATL54), a ubiquitin ligase associated with secondary cell wall formation. The ATL54 gene is co-expressed with secondary wall-associated genes, and the knock-out of ATL54 up-regulates the expression of cellulose, lignin, and xylan biosynthetic genes in apical stem portions of four-week-old plants. Here, we report the tissue-level localization patterns and the regulation of ATL54 expression. The beta-glucuronidase (GUS) reporter gene driven by the ATL54 promoter was significantly expressed in interfascicular fibers, xylary fibers, and vessels in inflorescence stems. A transient transfection assay using Arabidopsis T87 cells showed that the expression of the firefly luciferase gene driven by the ATL54 promoter was activated by MYB46, which is a key transcriptional activator of secondary wall formation. In addition, the electrophoretic mobility of ATL54 promoter fragments was shifted by a recombinant MYB46 protein. These results indicate that ATL54 expression is regulated by MYB46, and support the view that ATL54 has a role in secondary wall formation.

• 出版日期2013