Stabilization of novel immunoglobulin switch regions G-quadruplexes by naphthalene and quinoline-based ligands

作者：Carvalho Josue; Ferreira Joao; Pereira P; Coutinho E; Guedin Aurore; Nottelet Pierre; Salgado Gilmar E; Mergny Jean Louis; Queiroz J A; Sousa F; Cabrita Eurico J; Cruz C^*

来源：Tetrahedron, 2016, 72(9): 1229-1237.

DOI：10.1016/j.tet.2016.01.022

• G-quadruplex ligands
• Circular dichroism
• Thermal difference spectra
• FRET melting
• PCR-stop
• G4-FID

The topological study of novel quadruplex-forming DNA sequences found in immunoglobulin switch regions S mu and S gamma 3 is described along with the synthesis and characterization of naphthalene and quinoline-based ligands for the G-quadruplex binding and stabilization. Several biophysical and biochemical techniques are employed to assess the G-quadruplex formation and stabilization by the ligands. The results indicate that 58S gamma 3 folds into a parallel-stranded G-quadruplex structure in K+ buffer while 33S mu folds into a hybrid-type G-quadruplex in the same buffer. The novel naphthalene and quinoline-based ligands bind and stabilize specifically a variety of G-quadruplexes. This study provides evidence of the importance of G-quadruplex structures to the biology of immunoglobulin class switch recombination.

• 出版日期2016-3-3