Attomolar Protein Detection Using in-Hole Surface Plasmon Resonance

作者：Ferreira Jacqueline; Santos Marcos J L; Rahman Mohammad M; Brolo Alexandre G^*; Gordon Reuven; Sinton David; Girotto Emerson M

来源：Journal of the American Chemical Society, 2009, 131(2): 436-+.

DOI：10.1021/ja807704v

An in-hole nanohole surface plasmon resonance sensing scheme is demonstrated. Arrays of periodic nanoholes milled through thin layers of SiO(x) and gold were used to detect the binding of organic and biological molecules inside the nanoholes, while blocking the gold surfaces outside the holes. This new approach is more efficient than the previous nanohole array method, where the response was related to binding events taking place inside of the holes and on the top gold surface. The improved sensitivity to binding events and lower detection limit are related to resonant surface plasmon enhanced transmission through the arrays of nanoholes. The sensitivity was found to be 650nm/RIU and the detection of three attomoles of proteins was estimated from this scheme.

• 出版日期2009-1-21