Novel fibrinolytic enzymes from Virgibacillus halodenitrificans SK1-3-7 isolated from fish sauce fermentation

作者：Montriwong Aungkawipa; Kaewphuak Sasitorn; Rodtong Sureelak; Roytrakul Sittirak; Yongsawatdigul Jirawat^*

来源：Process Biochemistry, 2012, 47(12): 2379-2387.

DOI：10.1016/j.procbio.2012.09.020

• Fibrinolytic enzyme
• Virgibacillus halodenitrificans
• Fish sauce
• Subtilisin-like

Virgibacillus sp. SK1-3-7 exhibited the highest fibrinolytic activity among 25 bacterial isolates obtained from fish sauce fermentation. Results of 16S rRNA gene sequence analysis showed 99% homology to Virgibacillus halodenitrificans ATCC 49067. It was, therefore, identified as V. halodenitrificans SK1-3-7. Fibrinolytic enzymes from V. halodenitrificans SK1-3-7 were partially purified using ammonium sulfate fractionation, hydrophobic and ion-exchange chromatographies. The enzymes with molecular weight of 20- and 36-kDa showed fibrinolytic activity on a fibrin zymogram. The enzymes were stable between pH 4 and 10 and below 60 degrees C. The enzymes were activated by 20 mM CaCl2 and 0.15 M NaCl. The activity increased with CaCl2 up to 100 mM and increased with NaCl concentration up to 2M. In addition, the residual fibrinolytic activity of 61% was found at 4M NaCl. The enzymes were completely inhibited by phenylmethanesulfonyl fluoride (PMSF) and preferably hydrolyzed Suc-Ala-Ala-Pro-Phe-pNA, suggesting a subtilisin-like serine proteinase. V. halodenitrificans SK1-3-7 enzymes hydrolyzed fibrin to a greater extent than did plasmin. In addition, the enzymes were resistant to pepsin and trypsin digestion. The de nova peptide homology analysis of a 20- and 36-kDa proteinase revealed no matches to bacilli serine proteinases, suggesting that both were novel fibrinolytic enzymes.

• 出版日期2012-12