科研之友
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摘要
Partial cDNA sequences of three anthocyamin biosynthetic genes (FSH, flavanone 3-hydroxylase, DFR, dihydroflavonol 4-reductase, ANS, anthocyanidin synthase) were isolated from the petals of Camellia japonica. Then deduced partial amino acid sequences shared high homologies with those of woody plant species (CjF3Ha, 98.0%, CjF3Hb, 91.2% and CjDFR, 99.0% with Camellia sinensus, CjANS, 90.3% with Rhododendron x pulchrum). Some important amino acid residues for enzymatic activities were also conserved in the isolated clones, suggesting that the genes we identified in this study were the homologues of C. japonica. Gene-specific primer pairs were designed basect on each partial cDNA sequence. The application of these primer pairs to RT-PCR analyses tested.
- 出版日期2010-2
