A perfect functional marker for the gene of intermediate amylose content Wx-in in rice (Oryza sativa L.)

作者：Zhou, Lei^*; Chen, Shaoyu; Yang, Guocai; Zha, Wenjun; Cai, Haiya; Li, Sanhe; Chen, Zhijun; Liu, Kai; Xu, Huashan; You, Aiqing

来源：Crop Breeding and Applied Biotechnology, 2018, 18(1): 103-109.

DOI：10.1590/1984-70332018v18n1a14

• Intermediate amylose content
• waxy gene
• marker-assisted selection
• grain quality
• rice

The amylose content (AC) of rice is a key determinant of the grain cooking and eating quality. The Waxy (Wx) gene, which encodes granule-bound starch synthase I (GBSSI), is the major gene controlling AC in rice. The A-C substitution in exon 6 (Ex6A/C) alters the amino acid codon from serine to tyrosine and is associated with intermediate-AC rice (Wx-in). In this paper, we reported a perfect functional marker for the SNP Ex6A/C of Wx-in based on polymerase chain reaction with confronting two-pair primers (PCR-CTPP) in a single-tube PCR assay. Specificity and applicability of the PCR assay were verified in a Chinese mini core collection (MCC) of Oryza sativa L. and a breeding population. We recommend the use of the simple, inexpensive assay for routine genotyping for intermediate AC in the breeding population or discrimination of Wx-in genotypes in rice germplasm.

• 出版日期2018-3
• 单位湖北省农业科学院; 长江大学