Antimicrobial activity of the chloroform fraction of Drynaria fortunei against oral pathogens

作者：Cha Jeong Dan; Jung Eun Kyung; Choi Sung Mi; Lee Kyung Yeol; Kang Sung Wook

来源：Journal of Oral Science, 2017, 59(1): 31-38.

DOI：10.2334/josnusd.16-0150

• Drynaria fortunei
• oral bacteria
• synergistic effect
• antimicrobial activity
• minimum inhibitory concentrations
• minimum bactericidal concentrations

Drynaria fortunei (D. fortunei), widely used in traditional Korean medicine, is reportedly effective in treating inflammation, hyperlipidemia, bone fractures, oxidative damage, arteriosclerosis, rheumatism, and gynecological diseases. The objective of this study was to evaluate the antibacterial effects of the chloroform fraction of D. fortunei (DFCF) and assess the synergistic effects of DFCF with antibiotics against bacterial pathogens. This was carried out by calculating the minimal inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) and performing checkerboard dilution test and time-kill assays. The MICs/MBCs for DFCF, ampicillin, and gentamicin against all oral strains were >39-2,500/5,000 mu g/mL, 0.25-64/0.25-64 mu g/mL, and 0.5-256/1-512 mu g/mL, respectively. DFCF exhibited the highest activity against the periodontic pathogens Prevotella intermedia and Porphylomonas gingivalis. DFCF in combination with ampicillin showed a strong synergistic effect against oral bacteria (fractional inhibitory concentration (FIC) index <0.5), whereas on combining with gentamicin, it reduced the on half-eighth times than used alone (FICI < 0.5). DFCF combined with ampicillin or gentamicin killed 100% of most tested bacteria within 3-4 h. The results of this study demonstrate the antimicrobial and synergistic activity of DFCF and antibiotics against oral pathogens.

• 出版日期2017-3