Multiplex real-time RT-PCR for detecting chikungunya virus and dengue virus

作者：Pongsiri Piyathida; Praianantathavorn Kesmanee; Theamboonlers Apiradee; Payung**** Sunchai; Poovorawan Yong^*

来源：Asian Pacific Journal of Tropical Medicine, 2012, 5(5): 342-346.

DOI：10.1016/S1995-7645(12)60055-8

• Multiplex
• Real-time RT-PCR
• Chikungunya virus
• Dengue virus

Objective: To develop diagnostic test for detection chikungunya virus (CHIKV and Dengue virus (DENV) infection. Methods: We have performed a rapid, accurate laboratory confirmative method to simultaneously detect, quantify and differentiate CHIKV and DENV infection by single step multiplex real-time RT-PCR. Results: The assay%26apos;s sensitivity was 97.65%, specificity was 92.59% and accuracy was 95.82% when compared to conventional RT-PCR. Additionally, there was no cross reaction between CHIKV, DEW, Japanese encephalitis virus, hepatitis C. hepatitis A or hepatitis E virus. Conclusions: This rapid and reliable assay provides a means for simultaneous early diagnosis of CHIKV and DENV in a single-step reaction.

• 出版日期2012-5