An ultrasensitive electrochemical immunosensor based on C-60-modified polyamidoamine dendrimers and Au NPs for co-catalytic silver deposition

作者：Chen, Peipei; Wang, Ting; Zheng, Xiangli; Tian, Dong; Xia, Fangquan^*; Zhou, Changli^*

来源：New Journal of Chemistry, 2018, 42(6): 4653-4660.

DOI：10.1039/c8nj00059j

• SHAPED GOLD NANOPARTICLES
• NITROGEN-DOPED GRAPHENE
• ALPHA-FETOPROTEIN
• HYDROGEN-PEROXIDE
• SIGNAL TAG
• SENSOR
• IMMUNOASSAY
• FULLERENE-C-60
• OXIDE
• ACID

A convenient, highly efficient and ultrasensitive electrochemical immunosensor was developed using in situ deposited silver nanoparticles (Ag NPs) as tracing tags for fetoprotein (AFP) detection. The function of the immunosensor was achieved by a co-catalytic effect on the surface of nanocomposites containing polyamidoamine (PAMAM) dendrimers coated with C-60 (PAMAM-C-60 NPs) and gold nanoparticles (Au NPs). On the surface of Au@PAMAM-C-60 NPs labeled with an antibody (Ab(2)), via a sandwich-type immunoreaction, silver ions in solution were reduced to metallic Ag NPs. The electrochemical signal of the in situ deposited Ag NPs could be utilized to monitor the immunoreaction. Under optimum conditions, the peak current of the Ag NPs increased with increasing AFP concentration in the range from 0.1 pg mL(-1) to 10 ng mL(-1), with a detection limit of 0.03 pg mL(-1). Importantly, the designed electrochemical immunoassay strategy exhibited excellent sensitivity, stability, selectivity, and reproducibility.

• 出版日期2018-3-21
• 单位济南市环境保护监测站; 济南大学; 山东大学