Drug-containing serum of Xinfeng capsules protect against H9C2 from death by enhancing miRNA-21 and inhibiting toll-like receptor 4/phosphorylated p-38 (p-p38)/p-p65 signaling pathway and proin-flammatory cytokines expression

作者：Cao Yunxiang; Guo Yunke; Zong Ruikai; Huang Chuanbing; Wang Yue^*; Liu Jian^*

来源：Journal of Traditional Chinese Medicine, 2018, 38(3): 359-365.

DOI：10.1016/s0254-6272(18)30626-5

• Drug-containing serum
• Xinfeng capsules
• MicroRNAs
• Proinflammatory cytokines
• Toll-like receptor 4
• Myocardial cell
• Apoptosis

OBJECTIVE: To investigate effect of drug-containing serum of Xinfeng capsules on myocardial cell growth. @@@ METHODS: Drug-containing serum of Xinfeng capsules rat models were established by intragastricly administrated Xinfeng capsules. MTT assay was used to evaluated H9C2 cells viability. H9C2 cells were divided into-normal control group, triptolide group, lipopolysaccharide (LPS) group, drug-containing serum group and miRNA-21 inhibitor group. microRNA-21 (miRNA-21) inhibitor was structured and transfected into H9C2 cells. Western blot and immunofluorescence assay were applied to examine toll-like receptor 4 (TLR4), phosphorylated p-38 (p-p38) and p-p65 expression. Quantitative real-time PCR (qRT-PCR) was used to evaluated mRNA levels of miRNA-21. Enzyme linked immunosorbent (ELISA) was used to measure tumor necrosis factor a (TNF-alpha), IL-6 and IL-17 levels. @@@ RESULTS: Drug-containing serum treatment significantly increased cell viability compared to LPS treated group. qRT-PCR results indicated that miRNA-21 levels were significantly decreased in drug containing serum group compared to LPS group. Early and late apoptosis in drug-containing serum group were significantly decreased compared to LPS group. Western blot and immunofluorescence assay results showed that TLR4, p-p38 and p-p65 levels in drug-containing serum group were significantly decreased compared to LPS group. ELISA findings indicated that drug-containing serum significantly decreased inflammatory cytokine levels of TNF-alpha, IL-6 and IL-17. @@@ CONCLUSION: Drug-containing serum of Xinfeng capsules protect against lipopolysaccharide instructed H9C2 cells from death by enhancing miRNA21 and inhibiting TLR4/p-p38/p-p65 signaling pathway and proinflammatory cytokines expression.

• 出版日期2018-6
• 单位南京中医药大学; 安徽中医药大学