Augmentation of capsaicin-induced vasorelaxation of superior mesenteric artery (Capra hircus) in acidosis: role of TRPV1 channels

作者：Mohanty Ipsita; Nayak Nihar R; Parija Subas C^*

来源：Indian Journal of Traditional Knowledge, 2016, 15(4): 625-631.

• Acidosis
• Endothelial TRPV1
• Capsaicin
• Goat superior mesenteric artery

Capsaicin is known to induce vasorelaxation through activation of the transient receptor potential cation channel subfamily V member 1(TRPV1). However, TRPV1 is a nonselective cation channel and can be activated by a wide variety of exogenous and endogenous stimuli, including acidic conditions. The present study investigated the effect of changes in pH on capsaicin-induced vasorelaxation in both endothelium intact and denuded goat superior mesenteric artery (GSMA) rings, using a highly sensitive isometric force transducer. There was a significant increase in capsaicin-induced vasorelaxation in nor adrenaline (NA)-precontracted GSMA rings at acidic pHs of 6.8 (44.71 +/- 4.85%) and pH 6.0 (46.43 +/- 2.59%), compared to a physiological level of pH of 7.4 (33.25 +/- 2.62%). Although not completely abrogated, endothelium denudation and ruthenium red (RR) treatment significantly attenuated capsaicin-induced vasorelaxation at all pH levels. These data together suggest that, while other minor endothelium/TRVP1-independent mechanisms may exist, augmentation of capsaicin-induced vasorelaxation in acidosis is primarily endothelium-dependent and mediated through TRPV1 channels.

• 出版日期2016-10