A polydnaviral genome of Microplitis bicoloratus bracovirus and molecular interactions between the host and virus involved in NF-kappa B signaling

作者：Yu, Dong-Shuai; Chen, Ya-Bin; Li, Ming; Yang, Ming-Jun; Yang, Yang; Hu, Jian-Sheng; Luo, Kai-Jun^*

来源：Archives of Virology, 2016, 161(11): 3095-3124.

DOI：10.1007/s00705-016-2988-3

• SPODOPTERA-LITURA
• SEQUENCE
• NUCLEOPOLYHEDROVIRUS
• ORGANIZATION
• ACTIVATION
• SYSTEM
• RELISH
• ROLES
• MODEL

Polydnaviruses (PDVs) play a critical role in altering host gene expression to induce immunosuppression. However, it remains largely unclear how PDV genes affect host genes. Here, the complete genome sequence of Microplitis bicoloratus bracovirus (MbBV), which is known to be an apoptosis inducer, was determined. The MbBV genome consisted of 17 putative double-stranded DNA circles and 179 fragments with a total size of 336,336 bp and contained 116 open reading frames (ORFs). Based on conserved domains, nine gene families were identified, of which the I kappa B-like viral ankyrin (vank) family included 28 members and was one of the largest families. Among the 116 ORFs, 13 MbBV genes were expressed in hemocytes undergoing MbBV-induced apoptosis and further analyzed. Three vank genes (vank86, vank92, vank101) were expressed in hemocytes collected from Spodoptera litura larvae parasitized by M. bicoloratus, in which host NF-kappa B/I kappa Bs, including relish, dorsal, and cactus, were also persistently expressed. When Spli221 cells were infected with MbBV viral particles, mRNA levels of host and viral NF-kappa B/I kappa B genes were persistent and also varied in Spli221 cells undergoing virus-induced pre-apoptosis cell from 1 to 5 hours postinfection. Both were then expressed in a time-dependent expression in virus-induced apoptotic cells. These data show that viral I kappa B-like transcription does not inhibit host NF-kappa B/I kappa B expression, suggesting that transcription of these genes might be regulated by different mechanisms.

• 出版日期2016-11
• 单位上海人类基因组研究中心; 云南大学