Assessment of estradiol-induced gene regulation and proliferation in an immortalized mouse immature Sertoli cell line

作者：Kumar Narender; Srivastava Swati; Burek Malgorzata; Foerster Carola Y; Roy Partha^*

来源：Life Sciences, 2016, 148: 268-278.

DOI：10.1016/j.lfs.2016.01.027

• Estrogens
• Sertoli cell proliferation
• Estrogen receptors
• MAPK pathway
• MicroRNAs
• Epigenetic

Aims: The number of Sertoli cells during proliferative phase determines the fate of the germ cells in male reproductive system. Awell-characterized cell linemay help in better understanding of Sertoli cell biology. Hence, the present study assessed estradiol signaling in amouse immature Sertoli cell line (MSC-1) as an alternativemodel in place of primary culture of Sertoli cells.
Main methods: In this study, we used MSC-1 cell line, derived from 10-day old mice. The cell cycle parameters were assessed, and the expression and regulation of Sertoli cell-specific secretory genes (ABP; androgenbinding protein) and tight junction genes (claudin-5, occludin, and vimentin) in response to estradiol was studied.
Key findings: The results obtained suggested the presence of both estrogen receptors (ER alpha and ER beta) in MSC-1 cells. In vitro scratch assay and cell-cycle analysis suggested the proliferative effects of estradiol in both timeand dose-dependent manner. The gene expression profiles of ABP, claudin-5, and occludin showed biphasic regulation at lowand high doses of estradiol. Analysis of signaling pathways suggested the activation of extracellular signal-regulated kinase (ERK) pathway with significantly increased pERK/ERK ratio (p < 0.05). The results also suggested down regulation in the expression of mir-17 family members (mir-17, mir-20b, and mir-106a) (p < 0.05).
Significance: Considering the limited number of Sertoli cell lines and long-term survival inability of primary culture of Sertoli cells, MSC-1 cells could be a potential cell line for understanding themechanisms of various cellular events in Sertoli cells.

• 出版日期2016-3-1