An IRF-3 homolog that is up-regulated by DNA virus and poly I:C in turbot, Scophthalmus maximus

作者：Hu Guo Bin^*; Xia Jun; Lou Hui Min; Chen Xiao Ling; Li Jing; Liu Qiu Ming

来源：Fish & Shellfish Immunology, 2011, 31(6): 1224-1231.

DOI：10.1016/j.fsi.2011.07.011

• IRF-3
• Scophthalmus maximus
• TRBIV
• Gene expression
• IFN system

In this study, we described the structure, mRNA tissue distribution and regulation of an IRF-3 gene from turbot, Scophthalmus maximus (SmIRF-3). The gene sequence of SmIRF-3 is 6077 bp long, composed of 11 exons and 10 introns similar to known IRF-3 genes of fish, and encodes a peptide of 466 amino acids. The deduced protein sequence shares the highest identity of 56.0-81.2% with fish IRF-3 and possesses a DNA-binding domain (DBD), an IRF association domain (IAD) and a serine-rich domain (SRD) known to be important for the functions of IRF-3 in vertebrates. Phylogenetic analysis grouped SmIRF-3 with other IRF3s of vertebrates. SmIRF-3 transcripts were detectable in limited tissue types of healthy fish, with higher expression observed in head, kidney, spleen and kidney,. The SmIRF-3 was transcriptionally up-regulated by turbot reddish body iridovirus (TRBIV) and polyinosinic:polycytidylic acid (poly I:C) in the head kidney, spleen and gills, with showing a two wave induced expression during a 7-day time course in all cases. The highest inducibility and the likely earliest increase of SmIRF-3 expression were observed in the spleen, and poly I:C was a stronger inducer. In addition, the maximal expression level of SmIRF-3 arose prior to that of the Mx in all the cases.

• 出版日期2011-12
• 单位中国海洋大学