h0RFeome v3.1: A resource of human open reading frames representing over 10,000 human genes

作者：Lamesch Philippe; Li Ning; Milstein Stuart; Fan Changyu; Hao Tong; Szabo Gabor; Hu Zhenjun; Venkatesan Kavitha; Bethel Graeme; Martin Paul; Rogers Jane; Lawlor Stephanie; McLaren Stuart; Dricot Amelie; Borick Heather; Cusick Michael E; Vandenhaute Jean; Dunham Ian; Hill David E^*; Vidal Marc

来源：Genomics, 2007, 89(3): 307-315.

DOI：10.1016/j.ygeno.2006.11.012

• human ORFeome
• gateway system
• clone resource
• MGC collection
• nucleotide substitution rate
• OMIM
• GO slim
• visant
• hORFDB
• high-throughput cloning

Complete sets of cloned protein-encoding open reading frames (ORFs), or ORFeomes, tire essential tools for large-scale proteomics and systems biology studies. Here we describe human ORFeome version 3.1 (hORFeome v3.1), Currently the largest publicly available resource of full-length human ORFs (available at www.openbiosystems.com). Generated by Gateway recombinational cloning, this collection contains 12,212 ORFs, representing 10,214 human genes, and corresponds to a 51% expansion of the original hORFeome v1.1. An online human CRFeome database, hORFDB, was built and serves as the central repository for all cloned human ORFs (http://horfdb.dfci.harvard.edu). This expansion of the original ORFeome resource greatly increases the potential experimental search space for large-scale proteomics studies, which will lead to the generation of more comprehensive datasets.

• 出版日期2007-3