Dietary rosemary oil alleviates heat stress-induced structural and functional damage through lipid peroxidation in the testes of growing Japanese quail

作者：Turk Gaffari^*; Ceribasi Ali O; Simsek Ulku G; Ceribasi Songul; Guvenc Mehmet; Kaya Seyma Ozer; Ciftci Mehmet; Sonmez Mustafa; Yuce Abdurrauf; Bayrakdar Ali; Yaman Mine; Tonbak Fadime

来源：Animal Reproduction Science, 2016, 164: 133-143.

DOI：10.1016/j.anireprosci.2015.11.021

• Rosemary oil
• Heat stress
• Spermatogenic cell
• Testis
• Quail

Supplementation of natural antioxidants to diets of male poultry has been reported to be effective in reducing or completely eliminating heat stress (HS)-induced reproductive failures. In this study, the aim is to investigate whether rosemary oil (RO) has a protective effect on HS -induced damage in spermatozoa production, testicular histologic structures, apoptosis, and androgenic receptor (AR) through lipid peroxidation mechanisms ingrowing Japanese quail. Male chicks (n=90) at 15-days of age were assigned to two groups. The first group (n = 45) was kept in.a thermo-neutral (TN) room at 22 degrees C for 24 h/d. The second group (n =45) was kept in a room with a greater ambient temperature of 34 degrees C for 8 h/d (from 9:00 AM to 5:00 PM) and 22 degrees C for 16 h/d. Animals in each of these two groups were randomly assigned to three subgroups (RO groups: 0,125, 250 ppm), consisting of 15 chicks (six treatment groups in 2 x 3 factorial design). Each of subgroups was replicated three times with each replicate including five chicks. The HS treatment significantly reduced the testicular spermatogenic cell counts, amount of testicular Bcl-2 (anti-apoptotic marker) and amount of AR. In addition, it significantly increased testicular lipid peroxidation, Box (apoptotic marker) immunopositive staining, and the Bax/Bcl-2 ratio in conjunction with some histopathologic damage. Dietary supplementation of RO to diets of quail where the HS treatment was imposed alleviated HS -induced almost all negative changes such as increased testicular lipid peroxidation, decreased numbers of spermatogenic cells, and decreased amounts of Bcl-2 and AR, increased ratio of Bax/Bc1-2 and some testicular histopathologic lesion. In conclusion, dietary supplementation of RO for growing male Japanese quail reared in HS environmental conditions alleviates the HS -induced structural and functional damage by providing a decrease in lipid peroxidation.

• 出版日期2016-1