Anti-tumor activity of all-trans retinoic acid-incorporated glycol chitosan nanoparticles against HuCC-T1 human cholangiocarcinoma cells

作者：Chung Kyu Don; Jeong Young Il; Chung Chung Wook; Kim Do Hyung; Kang Dae Hwan^*

来源：International Journal of Pharmaceutics, 2012, 422(1-2): 454-461.

DOI：10.1016/j.ijpharm.2011.10.057

• All-trans retinoic acid
• Glycol chitosan
• Nanoparticles
• Cholangiocarcinoma
• Invasion

The aim of this study is to investigate antitumor activity of all-trans retinoic acid (RA)-incorporated glycol chitosan (GC) nanoparticles. RA-incorporated GC nanoparticles were prepared by electrostatic interaction between RA and amine group of GC. RA-incorporated GC nanoparticles have spherical shape and their particle size was 317 +/- 34.5 nm. They were simply reconstituted into aqueous solution without changes of intrinsic properties. RA-incorporated GC nanoparticles were evidently inhibited the proliferation of HuCC-T1 cholangiocarcinoma cells at higher than 20 mu g/ml of RA concentration while empty GC vegicles did not affect to the viablity of tumor cells. Apoptosis and necrosis analysis of tumor cells with treatment of RA or RA-incorporated GC nanoparticles also supported these results. Invasion test using Matrigel (R) also showed that invasion of tumor cells was significantly inhibited at higher than 20 mu g/ml of RA concentration. Wound healing assay also showed that RA-incorporated GC nanoparticles were inhibited migration of tumor cells as similar to RA itself. Our results suggested that RA-incorporated GC nanoparticles is a promising vehicles for RA delivery to HuCC-T1 cholangiocarcinoma cells.

• 出版日期2012-1-17