Distinguishing the dominant species of pathogen in maxillary sinusitis by sequencing DNA dataset analysis

作者：Zhang, Junyi; Li, Yunchuan^*; Lu, Xinxin; Wang, Xiangdong; Zang, Hongrui; Wang, Tong; Zhou, Bing; Zhang, Luo

来源：Gene, 2015, 561(2): 256-260.

DOI：10.1016/j.gene.2015.02.037

• Maxillary sinusitis
• Fungi
• Bacteria
• High-throughput sequencing

This study determined the microbial composition in maxillary sinusitis and determined the predominant fungal and bacterial species. Samples were obtained from 11 patients with fungal sinusitis and 2 healthy patients. The 13 samples were sequenced using an Illumina Hi-Seq 2000, and mapping to the human, bacterial, and the fungal genomes were based on several steps. Bioinformatics and statistical analyses were then performed for bacterial and fungal expressions in the samples. All samples were cultures for fungal growth. The sequencing data revealed that Aspergillus flavus and Aspergillus oryzae were the dominant fungal strains in the rhinosinusitis samples, and Bacillus cereus and Bacillus thuringiensis were the dominant bacterial strains. Indeed, the fungi and bacteria were associated with the development of fungal rhinosinusitis. Furthermore, B. cereus and B. thuringiensis may cooperate with Pseudomonas aeruginosa to inhibit the growth of fungal mycelia. Knowledge of the microbial composition can provide a diagnostic reference for patients with maxillary sinusitis.

• 出版日期2015-5-1
• 单位大庆油田总医院; 首都医科大学