Anti-skeletal muscle atrophy effect of Oenothera odorata root extract via reactive oxygen species-dependent signaling pathways in cellular and mouse model

作者：Lee Yong Hyeon; Kim Wan Joong; Lee Myung Hun; Kim Sun Young; Seo Dong Hyun; Kim Han Sung; Gelinsky Michael; Kim Tack Joong^*

来源：Bioscience Biotechnology and Biochemistry, 2016, 80(1): 80-88.

DOI：10.1080/09168451.2015.1075861

• Oenothera odorata
• heat shock protein 70
• superoxide dismutase
• muscle atrophy
• reactive oxygen species

Skeletal muscle atrophy can be defined as a decrease of muscle volume caused by injury or lack of use. This condition is associated with reactive oxygen species (ROS), resulting in various muscular disorders. We acquired 2D and 3D images using micro-computed tomography in gastrocnemius and soleus muscles of sciatic-denervated mice. We confirmed that sciatic denervation-small animal model reduced muscle volume. However, the intraperitoneal injection of Oenothera odorata root extract (EVP) delayed muscle atrophy compared to a control group. We also investigated the mechanism of muscle atrophy's relationship with ROS. EVP suppressed expression of SOD1, and increased expression of HSP70, in both H2O2-treated C2C12 myoblasts and sciatic-denervated mice. Moreover, EVP regulated apoptotic signals, including caspase-3, Bax, Bcl-2, and ceramide. These results indicate that EVP has a positive effect on reducing the effect of ROS on muscle atrophy.

• 出版日期2016-1-2