Hydrolysis of microalgal biomass using ruminal microorganisms as a pretreatment to increase methane recovery

作者：Barragan Trinidad Martin; Carrillo Reyes Julian; Buitron German^*

来源：Bioresource Technology, 2017, 244: 100-107.

DOI：10.1016/j.biortech.2017.07.117

• Ruminal fluid
• Microalgae
• Hydrolysis
• Pretreatment
• Methane

The use of ruminal fluid as a source of hydrolytic microorganisms for the pretreatment of a native consortium of microalgae (essentially Senedesmus) was investigated. The hydrolytic enzyme activity of the ruminal culture was first enriched in a bioreactor. Then, using the enriched culture, the effect of the microalgae to the ruminal fluid ratio (S/X) on the hydrolysis and subsequent production of methane was investigated. An S/X ratio of 0.5 showed the best hydrolysis efficiency (29%) reaching in a second stage process a methane yield of 193 mL CH4 g COD-1. The processing time (pretreatment plus methanization) was only 7 days. The predominant ruminal hydrolytic bacteria selected in the enrichment were principally Clostridium, Proteocatella and Pseudomonas.

• 出版日期2017-11