Analysis of HLA-G Polymorphisms in Couples with Implantation Failure

作者：Nardi Fabiola da Silva; Slowik Renata; Wowk Pryscilla Fanini; da Silva Jose Samuel; Gelmini Georgia Fernanda; Michelon Tatiana Ferreira; Neumann Jorge; Bicalho Maria da Graca^*

来源：American Journal of Reproductive Immunology, 2012, 68(6): 507-514.

DOI：10.1111/aji.12001

• Assisted reproduction treatment
• HLA-G
• soluble HLA-G
• implantation failure

Problem HLA-G expression is related as an immune modulator of fetalmaternal tolerance, and its levels was correlated with pregnancy outcome. In a casecontrol study, we investigate the association between the genetic variability of the HLA-G gene and serum levels of soluble HLA-G in cases of embryo implantation failure. Method of Study Forty couples with at least two unsuccessful fresh embryo transfers (implantation failure; IF) and 83 fertile couples with at least two successful pregnancies was genotyped by sequencing-based typing. HLA-G alleles were defined by nucleotide sequence variations at exon 2, 3, and 4, and the quantification of soluble HLA-G (sHLA-G) was performed by ELISA. Results There was a significant difference between the HLA-G allelic distributions between IF couples and the control couples. The HLA-G*01:03:01 allele was increased in the IF couples. There were no significant differences in the serum levels of sHLA-G in the IF and control groups. Conclusion The results suggest that the distribution of HLA-G products may play a significant role in the modulation of maternalfetal immune response.

• 出版日期2012-12