Anti-Human FLT3 Monoclonal Antibody That Inhibits Proliferation of Monocytic Leukemia Cell Line SHI-1

作者：Ju, Songguang; Xue, Zhimou; Ju, Songwen; Ge, Yan; Xie, Wei; Zhu, Huanting; Pan, Jianzhong; Zhang, Xueguang^*

来源：Hybridoma, 2011, 30(1): 61-67.

DOI：10.1089/hyb.2010.0073

• RECEPTOR TYROSINE KINASE
• MURINE FLT3
• LIGAND
• PROGENITORS

FLT3, a transmembrane molecule, was found on hematopoietic stem/progenitor cells and leukemia cells and determined to be a promising target in leukemia diagnosis and therapy. In this study a functional anti-human FLT3, monoclonal antibody (MAb) 10G6, was obtained and the specificity of this MAb was verified by flow cytometry. This MAb effectively recognized the FLT3 molecule expressed on a series of malignant cell lines. Furthermore, we demonstrated that MAb 10G6 inhibited the proliferation and migration ability and induced the apoptosis of SHI-1 cells that derived from a human monocytic leukemia. This functional anti-human FLT3 MAb provides a valuable tool for further study targeting the FLT3 on leukemia cells.

• 出版日期2011-2
• 单位苏州大学