A novel packing arrangement of AcrB in the lipid bilayer membrane

作者：Ly K; Bartho J D; Eicher T; Pos K M; Mitra A K^*

来源：FEBS LETTERS, 2014, 588(24): 4776-4783.

DOI：10.1016/j.febslet.2014.11.010

• AcrB
• Multi-drug efflux
• 2-D crystal
• Electron crystallography

The central component AcrB of the Escherichia coli drug efflux complex AcrA-AcrB-TolC has been extensively investigated by X-ray crystallography of detergent-protein 3-D crystals. In these crystals, AcrB packs as trimers - the functional unit. We visualized the AcrB-AcrB interaction in its native environment by examining E. coli lipid reconstituted 2-D crystals, which were overwhelmingly formed by asymmetric trimers stabilized by strongly-interacting monomers from adjacent trimers. Most interestingly, we observed lattices formed by an arrangement of AcrB monomers distinct from that in traditional trimers. This hitherto unobserved packing, might play a role in the biogenesis of trimeric AcrB.

• 出版日期2014-12-20