NMR solution structure of calerythrin, an EF-hand calcium-binding protein from Saccharopolyspora erythraea

作者：Tossavainen H; Permi P; Annila A; Kilpelainen I; Drakenberg T^*

来源：European Journal of Biochemistry, 2003, 270(11): 2505-2512.

DOI：10.1046/j.1432-1033.2003.03623.x

• calcium-binding
• calerythrin
• NMR
• structure

The structure of calerythrin, a prokaryotic 20 kDa calcium-binding protein has been determined by solution NMR spectroscopy. Distance, dihedral angle, J coupling, secondary chemical shift, residual dipolar coupling and radius of gyration restraints reveal four EF-hand motifs arranged in a compact globular structure. A tight turn in the middle of the amino acid sequence brings the two halves, each comprising a pair of EF-hands, close together. The structural similarity between calerythrin and the eukaryotic sarcoplasmic calcium-binding proteins is notable.

• 出版日期2003-6