A Multisite Performance Study Comparing the Reading of Immunohistochemical Slides on a Computer Monitor With Conventional Manual Microscopy for Estrogen and Progesterone Receptor Analysis

作者：Nassar Aziza^*; Cohen Cynthia; Ager**org Sally S; Zhou Weidong; Lynch Kathleen A; Barker Edward A; Vanderbilt Burton L; Thompson Jim; Heyman Eugene R; Olson Allen; Lange Holger; Siddiqui Momin T

来源：American Journal of Clinical Pathology, 2011, 135(3): 461-467.

DOI：10.1309/AJCP4VFKA5FCMZNA

• Breast cancer
• Digital images
• Estrogen receptor
• Progesterone receptor

A multisite study was conducted to assess the performance of the Aperio digital pathology system (Aperio Technologies, Vista, CA) for reading estrogen receptor (ER) and progesterone receptor (PR) slides on a computer monitor.
A total of 520 formalin-fixed breast tissue specimens were assayed at 3 clinical sites for ER and PR (260 each). Percentage and average staining intensity of positive nuclei were assessed. At each site, 3 pathologists performed a blinded reading of the glass slides using their microscopes initially and later using digital images on a computer monitor.
Comparable percentages of agreements were obtained for manual microscopy (MM) and manual digital slide reading (MDR) (ER, percentage of positive nuclei with cutoffs: MM, 91.3%-99.0%/MDR, 91.3%-100.0%; PR, percentage of positive nuclei with cutoffs: MM 83.8%-99.0%/MDR, 76.3%400.0%).
Reading ER and PR slides on a computer monitor using the Aperio digital pathology system is equivalent to reading the slides with a conventional light microscope.

• 出版日期2011-3