Alkaloids from Juglans Mandshurica maxim induce distinctive cell death in hepatocellular carcinoma cells

作者：Lou, Li-Li; Cheng, Zhuo-Yang; Guo, Rui; Yao, Guo-Dong^*; Song, Shao-Jiang^*

来源：Natural Product Research, 2019, 33(6): 911-914.

DOI：10.1080/14786419.2017.1413571

• Juglans mandshurica
• Alkaloids
• Hepatocellular carcinoma
• Apoptosis
• Autophagy

The aim of this work was to further investigate the anticancer potential of Juglans mandshurica Maxim, including the separation of active constituents and their anti-proliferative effects with underlying mechanism of action. Five alkaloids (1-5) were isolated from the bark of J. mandshurica. Among them, 1 showed the highest cytotoxic activities against Hep3B and HepG2 cells with an IC50 values of 61.80 and 56.24 mu M, respectively. Therefore, the cellular mechanism involved 1 was subsequently studied. Our results showed that 1 markedly caused apoptosis and autophagy, but without cell cycle arrest in HepG2 cells. Interestingly, only autophagic cell death was induced in 1-treated Hep3B cells. It is concluded that the isolated alkaloids exerted a certain anti-hepatoma potential, and our results may provide a basis for the further investigation of the alkaloids extracted from J. mandshurica.
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• 出版日期2019-3-19
• 单位沈阳药科大学