Determination of zearalenone and its metabolites in urine samples by liquid chromatography with electrochemical detection using a carbon nanotube-modified electrode

作者：de Andres Fernando; Zougagh Mohammed; Castaneda Gregorio; Rios Angel^*

来源：Journal of Chromatography A, 2008, 1212(1-2): 54-60.

DOI：10.1016/j.chroma.2008.09.112

• HPLC-electrochemical detection
• Carbon nanotubes
• Zearalenone
• Zearalenone metabolites
• Animal urine samples

A simple and sensitive method for the separation and determination of zearalenone (ZON) and its secondary metabolites, namely beta-zearalenol (beta-ZAL), beta-zearalanol (beta-ZOL), alpha-zearalenol (alpha-ZAL), alpha-zearalanol (alpha-ZOL) and zearalanone (ZAN) in urine (human, bovine and swine) samples is proposed. The method comprises previous clean-up and pre-concentration on C-18 adsorption cartridges of the analytes followed by liquid chromatographic separation and direct electrochemical detection at +0.85 mV using a carbon nanotube-modified glassy carbon electrode (CNT-GCE). This method allows the determination of beta-ZAL, beta-ZOL, alpha-ZAL, alpha-ZOL, ZAN and ZON in a linear range between 5 and 50ng mL(-1), with relative standard deviation values lower than 6.9% (intra-day) and 7.1% (inter-day), in all cases. Detection limits ranging between 1.3 ng mL(-1) (beta-ZOL, alpha-ZAL, alpha-ZOL) and 1.4 ng mL(-1) (beta-ZAL, ZAN, ZON) were achieved. The usefulness of the proposed method was demonstrated by the analysis of spiked and natural samples of human, bovine and swine urine samples.

• 出版日期2008-11-28