Mitochondrial DNA sequences of greenbug (Homoptera : Aphididae) biotypes

作者：Aikhionbare FO^*; Mayo ZB

来源：Biomolecular Engineering, 2000, 16(6): 199-205.

DOI：10.1016/S1389-0344(99)00054-4

• greenbug
• biotype
• mtDNA
• cytb gene
• ND genes
• rRNA genes

Sequence comparisons were made for 738-bp of mtDNA cloned from seven greenbug, Schizaphis graminum, biotypes (B, C, E, F, G, H and I) obtained from laboratory colonies maintained by USDA-ARSI Stillwater, OK. These sequences include parts of the genes for 16S ribosomal subunit (16S rRNA), tRNA(leu), tRNA(ser), cytochrome b (cytb) and NADH dehydrogenase (ND) subunits one and four. Sequence data revealed considerable variation in 86 (12%) nucleotide sites over the 738-bp sequenced among the seven greenbug biotypes. Nucleotide invariance was observed within the seven greenbug biotypes from both the laboratory colonies and field collected biotype E greenbugs from Kansas, Nebraska, Oklahoma, and Texas. Published by Elsevier Science B.V.

• 出版日期2000-6