Association of promoter methylation of ER alpha and ER beta with sporadic breast cancer-a study from North India

作者：Chattopadhyay Shilpi; Deo S V S; Shukla N K; Husain Syed Akhtar^*

来源：Tumor Biology, 2014, 35(8): 7911-7919.

DOI：10.1007/s13277-014-2052-4

• Breast cancer
• Estrogen receptors
• DNA methylation
• Protein expression

Methylations in estrogen receptor (ER) alpha and ER beta are known to be involved in the pathogenesis of breast cancer. Here, we explore the role of promoter methylation of estrogen receptors, ER alpha and ER beta, in sporadic breast cancer cases from a North Indian population. To this end, association between ER alpha and ER beta methylation status along with different clinicopathological parameters and its correlation with protein expression was examined. Four hundred eighty paired breast cancer tissue samples and adjacent normal controls from 240 sporadic breast cancer patients were included, and their clinical and demographic profiles were recorded. ER alpha and ER beta methylation was determined by methylation-specific polymerase (MSP) chain reaction. Our findings demonstrate that methylation of ER alpha and ER beta occurs in high frequency and appears to be a mechanism of gene silencing in our population. Furthermore, on performing stratified analysis, we observed strong associations between ER alpha/ER beta methylation and ER, PR, and HER2 status, tumor size, clinical stage, and triple negative tumors. Thus, our study not only highlights the role of ER alpha/ER beta methylation in breast cancer but also suggests the ER alpha/ER beta methylation pattern as a biomarker for assessing breast cancer risk.

• 出版日期2014-8
• 单位河北医科大学