Development of multiplex real-time reverse-transcriptase polymerase chain reaction assay for simultaneous detection of Zika, dengue, yellow fever, and chikungunya viruses in a single tube

作者：Wu, Wenhui; Wang, Jin; Yu, Nan; Yan, Juying; Zhuo, Zhihao; Chen, Mengyuan; Su, Xiaosong; Fang, Mujin; He, Shuizhen; Zhang, Shiyin^*; Zhang, Yanjun^*; Ge, Shengxiang^*; Xia, Ningshao

来源：Journal of Medical Virology, 2018, 90(11): 1681-1686.

DOI：10.1002/jmv.25253

• chikungunya
• dengue
• multiplex real-time reverse-transcriptase polymerase chain reaction
• yellow fever
• Zika

Zika virus (ZIKV), dengue virus (DENV), chikungunya virus (CHIKV) and yellow fever virus (YFV) share the same mosquito vectors and have similar clinical manifestations early stage of infection. Therefore, simultaneously differentiating these viruses from each other is necessary. We developed a multiplex real-time reverse-transcriptase polymerase chain reaction (RT-PCR) assay for the differentiation of these four viruses in a single tube. The linear range was established by regression analysis, and the R-2 value for each viruswas 0.98, and the 95% lower limit of detectionfor each virus was as follows (copies/reaction): ZIKV-Asian, 9; ZIKV-Africa, 15; CHIKV, 11; DENV-1, 19; DENV-2, 13; DENV-3, 24; DENV-4, 36; and YFV, 17. Meanwhile, our multiplex real-time RT-PCR has a good consistency with the commercial singleplex assay. In summary, the developed assay can be effectively used for the diagnosis of ZIKV, DENV, CHIKV, and YFV infections.

• 出版日期2018-11
• 单位厦门大学; 南方医科大学; 浙江省疾病预防控制中心