Anti-metastatic and anti-angiogenic activities of sulfated polysaccharide of Sepiella maindroni ink

作者：Zong Aizhen; Zhao Ting; Zhang Yan; Song Xinlei; Shi Yikang; Cao Hongzhi; Liu Chunhui; Cheng Yanna; Qu Xianjun; Cao Jichao; Wang Fengshan^*

来源：Carbohydrate Polymers, 2013, 91(1): 403-409.

DOI：10.1016/j.carbpol.2012.08.050

• Sulfated polysaccharide
• Sepiella maindroni ink
• ICAM-1
• Angiogenesis
• Melanoma metastasis

A previous study demonstrated that SIP-SII, a sulfated Sepiella maindroni ink polysaccharide, suppressed the invasion and migration of cancer cells via the inhibition of the proteolytic activity of matrix metalloproteinase-2 (MMP-2). Therefore, this study investigated the anti-metastatic effect of SIP-SII in vivo. SIP-SII (15 and 30 mg/kg d) markedly decreased B16F10 pulmonary metastasis in mice models by 85.9% and 88.0%, respectively. Immunohistochemistry showed that SIP-SII decreased the expression of the intercellular adhesion molecule 1 (ICAM-1) and basic fibroblast growth factor (bFGF) in lung metastasis nodules. In addition, SIP-SII inhibited neovascularization in chick chorioallantoic membrane assay at 0.08-2 mg/mL. In the in vitro experiments, SIP-SII (0.8-500 mu g/mL) significantly decreased the protein and mRNA expression of ICAM-1 and bFGF in SKOV3 and EA.hy926 cells, respectively. These results suggested that SIP-SII might suppress melanoma metastasis via the inhibition of the tumor adhesion mediated by ICAM-1 and the angiogenesis mediated by bFGF, as well as resulting in depression of the invasion and migration of carcinoma cells.

• 出版日期2013-1-2
• 单位山东大学