A Study on Repair of Porcine Articular Cartilage Defects With Tissue-Engineered Cartilage Constructed In Vivo By Composite Scaffold Materials

作者：Lin, Pan B.; Ning, Li J.^*; Lian, Qin Z.; Xia, Zhao; Xin, Yang; Sen, Bi H.; Fei, Nie F.

来源：Annals of Plastic Surgery, 2010, 65(4): 430-436.

DOI：10.1097/SAP.0b013e3181d6e38b

• cartilage microparticle acellular tissue matrix
• acellular cartilage
• fibrin glue
• injectable tissue-engineered cartilage

The study was performed to find out a promising injectable composite scaffold for cartilage tissue engineering. By using a composite of allogenous cartilage microparticle acellular tissue matrix (CMACTM) and fibrin glue (Fg) as injectable scaffold materials, tissue-engineered cartilage was constructed in vivo, and the effects of which on the repair of porcine articular cartilage defects were observed. @@@ CMACTM was obtained from domestic pigs. The chondrocytes were prepared from experimental mini-type pigs and expanded in vitro. Fg was used as a scaffold material. The composite of CMACTM, second-passage chondrocytes, and Fg was replanted to the articular cartilage defective regions in autologous mini-type pig by injection. At 12 weeks after replantation, samples were collected and analyzed by general observation and histologic staining. @@@ The constructed tissue-engineered cartilage exhibited a good efficiency in the repair of articular cartilage defects. Cells in the constructed tissue-engineered cartilage grew well and were able to secrete cartilaginous matrix. The tissue-engineered cartilage showed a better biologic performance than the control. @@@ A composite of allogenous CMACTM and Fg was a promising injectable scaffold for cartilage tissue engineering, which could be used to repair articular cartilage defects by a minimally invasive procedure.

• 出版日期2010-10
• 单位北京大学