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摘要
<jats:p>Structural changes contribute to airway hyperresponsiveness and airflow obstruction in asthma. Emerging evidence points to the involvement of c-kit<jats:sup>+</jats:sup> cells in lung homeostasis, although their potential role in asthma is unknown. Our aim was to isolate c-kit<jats:sup>+</jats:sup> cells from normal mouse lungs and to test whether these cells can interfere with hallmarks of asthma in an animal model. Adult mouse GFP-tagged c-kit<jats:sup>+</jats:sup> cells, intratracheally delivered in the ovalbumin-induced airway hyperresponsiveness, positively affected airway remodeling and improved airway function. In bronchoalveolar lavage fluid of cell-treated animals, a reduction in the number of inflammatory cells and in IL-4, IL-5, and IL-13 release, along with an increase of IL-10, was observed. In MSC-treated mice, the macrophage polarization to M2-like subset may explain, at least in part, the increment in the level of anti-inflammatory cytokine IL-10. After in vitro stimulation of c-kit<jats:sup>+</jats:sup> cells with proinflammatory cytokines, the indoleamine 2,3-dioxygenase and TGF<jats:italic>β</jats:italic> were upregulated. These data, together with the increased apoptosis of inflammatory cells in vivo, indicate that c-kit<jats:sup>+</jats:sup> cells downregulate immune response in asthma by influencing local environment, possibly by cell-to-cell contact combined to paracrine action. In conclusion, intratracheally administered c-kit<jats:sup>+</jats:sup> cells reduce inflammation, positively modulate airway remodeling, and improve function. These data document previously unrecognized properties of c-kit<jats:sup>+</jats:sup> cells, able to impede pathophysiological features of experimental airway hyperresponsiveness.</jats:p>
- 出版日期2016
