EXTRACTION OF gamma-ORYZANOL FROM RICE BRAN

作者：Heidtmann Bemvenuti Renata^*; Nora Nathali Saiao; Badiale Furlong Eliana

来源：Ciencia e Agrotecnologia, 2012, 36(6): 665-673.

• Antioxidant
• spectrophotometer
• stability
• phytochemistry

gamma-oryzanol, a phytochemical, has antioxidant activities and potential health benefits. In this study, we aimed to develop a method for extracting high amounts of gamma-oryzanol from rice bran and for verifying its stability in different solvents. Of the 5 methods under investigation, the one that yielded the highest gamma-oryzanol content was further improved by using a central composite rotational design (DCCR - 2(4), including 8 tests in axial conditions and 4 central points, totaling 28 trials). The parameters under study were temperature, time, volume, and the ratio of hexane: isopropanol. High gamma-oryzanol content (13.98 mg.g(-1)) was obtained by using the following extraction conditions: 40 degrees C, 40 min, and 75 mL hexane: isopropanol (1:3). The limits of detection and quantification of the method were 0.9 mu g g(-1) and 31 mu g g(-1), respectively. The instrumental precision was 0.004%, the repeatability (CVr) was 9.4%, and the recovery was 111.7 +/- 17.7%. gamma-oryzanol is more stable in isopropanol than in hexane, in which 100% concentration could be maintained after refrigeration for 72 days.

• 出版日期2012-12