科研之友
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摘要
o-Phenylphenol is generally utilized as a disinfectant for citrus fruits. The purpose of this study is to develop a high-performance liquid chromatography coupled with ultraviolet detection (380 nm) method for simultaneous determination of o-phenylphenol, and its analogues (m-phenylphenol and p-phenylphenol) in grapefruit juice after pre-column derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F). 2-Hydroxyfluorene was used as an internal standard (IS). Standard curves were obtained after derivatization with NBD-F in borate buffer (pH 8.0) at room temperature for 5 min. The three NBD-F derivatives were almost completely separated on a Cholester column (5 mu m, 3.0 mm i.d. x 150 mm). Calibration plots were linear in the range of absolute amount of 1.04 2.08 to 41.6 ng/50 mu L injection volume, with r (2) values a parts per thousand yen0.9981, for the three compounds. The lower limits of detection were 0.3 to 0.7 ng/50 mu L injection volume (signal-to-noise ratio of 3 : 1). The coefficients of variation were less than 11.1%. After extraction of grapefruit juice (2.0 mL) with n-pentane, the level of o-phenylphenol in the juice was estimated to be 20.2 +/- 2.0 ng/mL (n = 6, mean +/- SD), while m-phenylphenol and p-phenylphenol were below the lower limits of quantification. The recovery values of the three phenylphenols from samples spiked with a standard mixture of authentic compounds and IS were satisfactory (99.1 to 118.7%).
- 出版日期2015-3
