科研之友
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摘要
Leuciscus merzbacheri is a unique vulnerable indigenous fish which is only distributed in the Junggar Basin, Xinjiang. In this study, researchers cloned two L. merzbacheri 汕-actin promoter fragments of different length: SZ11 and SZ21 and analyzed their structural features. The mammalian expression vector pEGFP-N1 was used to construct eukaryotic expression vectors 汕1 pEGFP-N1-AFP III and 汕2 pEGFP-N1-AFP III in which fish type III antifreeze protein gene was used as the structural gene. The results showed that the cloned two fragments of 汕-actin promoters had the ability to drive the expression of the green fluorescent protein gene in BHK-21 cells. These data suggest that the vectors researchers constructed based on 汕-actin promoter could be exploited as all fish recombinant eukaryotic expression vector.
- 出版日期2012
