Antimalarial and free radical scavenging activities of rhizomes of Polygonatum verticillatum supported by isolated metabolites

作者：Khan Haroon; Saeed Muhammad^*; Khan Murad Ali; Khan Inamullah; Ahmad Manzoor; Muhammad Naveed; Khan Afsar

来源：Medicinal Chemistry Research, 2012, 21(7): 1278-1282.

DOI：10.1007/s00044-011-9637-x

• Polygonatum verticillatum
• Antimalarial
• Antioxidant
• Cytotoxicity
• HMF
• Diosgenin

Antimalarial activity of the crude extract of Polygonatum verticillatum rhizomes and its sequentially partitioned fractions were investigated against Plasmodium falciparum. The crude extract possessed notable activity (IC50: 21.67 mu g/mL) that enhanced reasonably upon fractionation. The antiparasitic potency of the n-hexane fraction was maximum (IC50: 2.33 mu g/mL) followed by chloroform (IC50: 4.62 mu g/mL). However, the remaining fractions showed insignificant activity in the assay. The extracts of the plant showed marked scavenging activity on stable free radical, DDPH. The most potent antioxidant was the chloroform fraction (IC50: 90 mu g/mL) followed by ethyl acetate (IC50: 93 mu g/mL) and n-butanol (IC50: 95 mu g/mL) fractions. In the brine shrimps lethality test, the extracts were found nontoxic with the exception of ethyl acetate fraction (LD50: 492.846 mu g/mL). The bioactivity-guided isolation resulted into 5-hydroxymethyl-2-furaldehyde (HMF) and diosgenin which strongly supports the present experimental findings.

• 出版日期2012-7