Membrane associated protein flotillin-2 in Litopenaeus vannamei plays a role in WSSV infection

作者：Shi, Hong; Guo, Guangran; Liu, Rongdiao; Wang, Chuanqi; Xu, Xun; Ruan, Lingwei^*

来源：Fish & Shellfish Immunology, 2016, 54: 247-253.

DOI：10.1016/j.fsi.2016.04.010

• Shrimp
• Flotillin-2
• WSSV
• Endocytosis

Flotillin-2, an important protein of vesicular endocytosis, plays an essential role in a large number of cellular processes, including viruses and pathogen infection. In the present study, a flotillin-2 homolog in Litopenaeus vannamei, designed as Lvflotillin-2, was cloned and characterized. To analyze the putative role of Lvflotillin-2 during white spot syndrome virus (WSSV) infection, real-time quantitative PCR was performed. The result showed that the transcriptional level of Lvflotillin-2 was up-regulated significantly after virus challenge. Furthermore, upon WSSV stimulation, Lvflotillin-2 in shrimp cells could translocate from the plasma membrane to intracellular compartments, and unexpectedly, also into nucleus. Additionally, depletion of Lvflotillin-2 inhibited WSSV gene ie1 transcription. It suggested that Lvflotillin-2 could be hijacked by WSSV. These observations indicated that Lvflotillin-2 was involved in WSSV infection, and presented here should be useful for gaining insight into shrimp immunity and WSSV pathogenesis.

• 出版日期2016-7
• 单位厦门大学; 国家海洋局第三海洋研究所