Autophagy is decreased in mesenteric fat tissue but not in intestinal mucosae of patients with Crohn%26apos;s disease

作者：Leal Raquel F^*; Coy Claudio S R; Velloso Licio A; Dalal Sushila; Portovedo Mariana; Rodrigues Viviane S; Coope Andressa; Ayrizono Maria L S; Fagundes Joao J; Milanski Marciane

来源：Cell and Tissue Research, 2012, 350(3): 549-552.

DOI：10.1007/s00441-012-1491-8

• Crohn%26apos
• s disease
• Inflammatory bowel disease
• Autophagy
• Cytokines
• Mesenteric fat

Crohn%26apos;s disease (CD) is a chronic intestinal disease with a multifactorial etiology. Recently, a role for mesenteric fat has been proposed in CD pathophysiology, since fat hypertrophy is detected close to the affected intestinal area; however, there are few studies regarding autophagy and the hypertrophied mesenteric tissue in CD. To evaluate autophagy-related proteins in intestinal mucosae and mesenteric fat of patients with CD and controls, patients with ileocecal CD (CD Group) and with non-inflammatory disease (FC Group) selected for surgery were studied. Expression of LC3-II was determined by immunoblotting of protein extracts. In addition, beclin-1, LC3 and Atg16-L1 RNA levels were measured using RT-PCR. The expression of LC3-II was significantly lower in the mesenteric tissue and higher in intestinal mucosae of CD when compared to controls. However, mRNA expression of autophagy-related proteins was similar when comparing the mesenteric fat groups. These findings suggest a defect in autophagy activation in the mesenteric fat tissue of CD individuals, which could be involved in the maintenance of the inflammatory process.

• 出版日期2012-12