摘要
Individual casein fractions are of growing interest because of their multifunctional applications. The fractions of caseins (alpha(S)-, beta- & kappa-) possess a wide range of bio- and techno-functional properties. Although various isolation and purification methods to obtain casein fractions have been reported, there is still a need for improvement, especially on a technical scale. The aim of this study was to develop and establish a continuous process for the fractionation of caseins. The fractions were obtained from micellar casein by means of selective precipitation. The separation process was performed using a temperature-controlled decanter centrifuge. Enrichment of the fractions was optimised by altering the operational parameters of the decanter (inner weir diameter (empty set(weir)), centrifugal force (F-Z), differential speed (Delta n), flow rate (V.)). A 95% pure casein fraction containing alpha(S)- and beta- casein was obtained. The purity and yield of the kappa-casein fraction were 54% and 83%, respectively.
- 出版日期2018-9