A digital microfluidic interface between solid-phase microextraction and liquid chromatography-mass spectrometry

作者：Choi Kihwan; Boyaci Ezel; Kim Jihye; Seale Brendon; Barrera Arbelaez Luis; Pawliszyn Janusz; Wheeler Aaron R^*

来源：Journal of Chromatography A, 2016, 1444: 1-7.

DOI：10.1016/j.chroma.2016.03.029

• Digital microfluidics
• Solid-phase microextraction
• HPLC-MS
• SPME
• Urine
• Steroid
• Hormone

We introduce a method to couple solid-phase microextraction (SPME) with HPLC-MS using digital microfluidics (DMF). In the new system, SPME fibers are used to extract analytes from complex sample solutions, after which the analytes are desorbed into solvent droplets in a DMF device. The open geometry of DMF allows straightforward insertion of SPME fibers without requiring a complicated interface, and automated droplet manipulation enables multiplexed processing of the fibers. In contrast to other multiplexed SPME elution interfaces, the low volumes inherent to DMF allow for pre-concentration of analytes prior to analysis. The new SPME-DMF-HPLC-MS method was applied to the quantification of pg/mL-level free steroid hormones in urine. We propose that this new method will be useful for a wide range of applications requiring cleanup and pre-concentration with convenient coupling to high-performance analytical techniques.

• 出版日期2016-4-29