Diversity of culturable moderately halophilic and halotolerant bacteria in a marsh and two salterns a protected ecosystem of Lower Loukkos (Morocco)

作者：Berrada Imane; Willems Anne; De Vos Paul; El Fahime ElMostafa; Swings Jean; Bendaou Najib; Melloul Marouane; Amar Mohamed^*

来源：African Journal of Microbiology Research, 2012, 6(10): 2419-2434.

DOI：10.5897/AJMR-11-1490

• Halophilic bacteria
• Lower Loukkos
• sediment
• biodiversity
• 16S rRNA gene sequencing
• hydrolytic enzymes

To study the biodiversity of halophilic bacteria in a protected wetland located in Loukkos (Northwest, Morocco), a total of 124 strains were recovered from sediment samples from a marsh and salterns. 120 isolates (98%) were found to be moderately halophilic bacteria; growing in salt ranges of 0.5 to 20%. Of 124 isolates, 102 were Gram-positive while 22 were Gram negative. All isolates were identified based on 16S rRNA gene phylogenetic analysis and characterized phenotypically and by screening for extracellular hydrolytic enzymes. The Gram-positive isolates were dominated by the genus Bacillus (89%) and the others were assigned to Jeotgalibacillus, Planococcus, Staphylococcus and Thalassobacillus. The Gram negative isolates were dominated by the genus Vibrio (41%) and the others were assigned to Halomonas, Psychrobacter, Marinobacterium, Pseudoalteromonas, Salinivibrio and Photobacterium. The growth of strains obtained under different physico-chemical conditions and the screening for hydrolytic enzymes showed a high diversity even within the same species.

• 出版日期2012-3-16