Detection of SJNNV and RGNNV genotypes using a relative quantification RT-PCR assay

作者：Cherif N^*; Lopez Jimena B; Garcia Rosado E; Cano I; Castro D; Borrego J J; Alonso M C; Hammami S

来源：Journal of Applied Ichthyology, 2011, 27(3): 805-812.

DOI：10.1111/j.1439-0426.2010.01592.x

P>Viral Encephalopathy and Retinopathy (VER), is caused by a nodavirus included within the Betanodavirus genus of the Nodaviridae family. This disease affects more than 30 marine fish species worldwide and has been a major obstacle in the aquaculture industry; control of the disease is based on virus detection, essentially in carrier specimens. This study describes a real time PCR procedure for viral nervous necrosis virus detection from several organs of sea bass, Senegalese sole, and gilt-head sea bream, from fish displaying either clinical symptoms or asymptomatic cases. The sensitivity of this technique was about 106-fold higher than that of the conventional RT-PCR. The newly designed primers detected nodavirus isolates belonging to the RGNNV and SJNNV genotypes.

• 出版日期2011-6