Derivatization for liquid chromatography-mass spectrometry

作者：Qi Bao Ling; Liu Ping; Wang Qiu Yi; Cai Wen Jing; Yuan Bi Feng^*; Feng Yu Qi

来源：TrAC-Trends in Analytical Chemistry, 2014, 59: 121-132.

DOI：10.1016/j.trac.2014.03.013

• Derivatization
• Environmental analysis
• Food-safety evaluation
• Liquid chromatography
• Mass spectrometric imaging
• Mass spectrometry
• Metabolite
• Peptide
• Pharmaceutical analysis
• Protein

Liquid chromatography-mass spectrometry (LC-MS) is one of the most prominent analytical techniques, due to its inherent selectivity and sensitivity. In LC-MS, chemical derivatizations are frequently used to enhance the MS ionization efficiency and selectivity, to facilitate structure elucidation, and to improve the chromatographic separation. In this review, we present an overview of derivatization-based LC-MS analysis. We summarize the reaction mechanisms of representative derivatization reagents and the selection strategy to guide and to stimulate future studies. Furthermore, we emphasize applications of derivatization in peptide and protein analysis, metabolite analysis, environmental analysis, pharmaceutical analysis, food-safety evaluation and MS imaging.

• 出版日期2014-8
• 单位武汉大学