Antigenotoxic Potential of Gymnema montanum Leaves on DNA Damage in Human Peripheral Blood Lymphocytes and HL-60 Cell Line

作者：Ramkumar K M; Sankar L; Manjula C; Krishnamurthi K; Devi S Saravana; Chakrabarti T; Kalaiselvi K; Palanivel M; Rajaguru P^*

来源：Environmental and Molecular Mutagenesis, 2010, 51(4): 285-293.

DOI：10.1002/em.20543

• comet assay
• human lymphocytes
• Gymnema montanum
• genotoxicity
• total phenolics
• HL-60 cell line

In this study we have evaluated the genoprotective effect of the ethanol extract of Gymnema montanum (GLEt) leaves in human peripheral blood lymphocytes and HL-60 cell line in vitro using the comet assay. DNA damage was induced by treating the cells with H(2)O(2) and methyl methane sulphonate (MMS). GLEt treatment effectively protected the lymphocytes and HL-60 cell line from H(2)O(2)-induced oxidative DNA damage in a dose-dependent manner whereas it was not effective against alkylative DNA damage caused by MMS. The global percent repair efficiency also showed that both pre- and post- GLEt treatment provided effective protection against H(2)O(2) induced DNA damage but not as effective against MMS. At 200 mu g ml(-1) level, its repair capacity against H(2)O(2) induced DNA damage was comparable to that of vitamin-C (100 mu M). Furthermore, exposure to GLEt reduced the formation of apoptotic cells caused by H(2)O(2), which was demonstrated by the decreased sub-G1-DNA content in cell cycle analysis and apoptotic frequencies of lymphocytes in an annexin-V binding assay. In addition, GLEt was found to have effective peroxide scavenging ability in dose-dependent manner. The protective efficiency of the extract was found to be directly proportional to its total phenolic content. The present study indicates that G. montanum leaves are a significant source of phytochemicals with antigenotoxic and antioxidant activity, and thus has potential therapeutic use. Environ. Mol. Mutagen. 51:285-293, 2010.

• 出版日期2010-5