Physicochemical properties and prebiotic activities of polysaccharides from longan pulp based on different extraction techniques

作者：Huang, Fei; Liu, Huijun; Zhang, Ruifen; Dong, Lihong; Liu, Lei; Ma, Yongxuan; Jia, Xuchao; Wang, Guangjin; Zhang, Mingwei^*

来源：Carbohydrate Polymers, 2019, 206: 344-351.

DOI：10.1016/j.carbpol.2018.11.012

• Longan polysaccharides
• Different extraction methods
• Glycosidic linkage
• Probiotic proliferation

Longan pulp polysaccharides were extracted with hot water (LP-H), superfine grinding (LP-S) and superfine grinding-assisted enzymatic treatments (LP-SE). The yields, physicochemical properties and prebiotic activities of polysaccharides were investigated. Compared with LP-H and LP-S, the yield, sugar content, solubility, arabinose and mannose percentage of LP-SE increased while its apparent viscosity, particle size, Mw and glucose percentage declined. Three LPs contained similar glycosidic linkage of -> 3)alpha-L-Araf-(1 ->, -> 3,6)-beta-D-Galp-(1 -> and alpha-L-Rhap(1 ->, while they each contained specific glycosidic linkage of -> 4)-beta-D-Glcp(1 ->, -> 4)-beta-D-Galp-(1 -> and -> 5)-alpha-L-Araf-(1 -> in LP-H, LP-S and LP-SE, respectively. Moreover, LP-SE exhibited stronger stimulation than LP-H and LP-S on the proliferation of Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus fermentum and Leuconostoc mesenteroides. The results indicated three extraction methods had some effect on chemical composition and structure of polysaccharide. LP-SE extracted by superfine grinding-assisted enzymatic treatment exhibited the highest prebiotic activities, which have a great potential in applying in functional food and medical industry.

• 出版日期2019-2-15
• 单位湖北工程学院; 广东省农业科学院