摘要
A novel rod-shaped, Gram-stain-negative, non-gliding and aerobic strain surrounded by a multilayer capsule, designated 4-1-2(T), was isolated from a till sample of Collins glacier front, Antarctica. The bacterium formed yellow, circular, convex and smooth colonies. Growth occurred at 4-28 degrees C (optimuml8-20 degrees C), at pH 7.0-10.0 (optimum pH 9.0) and with 0-1 % NaCI. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 4-T-2(T) belonged to the genus Flavobacterium. Strain 4-T-2T shared the highest 16S rRNA gene sequence similarity with the type strain of Flavobacterium algicola (96.7 %). The DNA G + C content of strain 4-T-2(T) was 36.2 mol%. The only menaquinone was MK-6. The major cellular fatty acids were iso-C-15 (: 0,) iso-C-15 (:) (1), iso-C-16 (:) (0) and summed feature 9 (comprising iso-C-17 : (1)omega 9c and/or 10-methyl C-16 : 0). Polar lipid profile consisted phosphatidylethanolamine, one unidentified aminolipid, one unidentified aminophospholipid and one unidentified lipid. On the basis of phylogenetic, physiological and chemotaxonomic data, strain 4-T-2(T) is considered to represent a novel species of the genus Flavobacterium, for which the name Flavobacterium collinsense sp. nov. is proposed. The type strain is 4-T-2(T) (=CCTCC AB 2014004T=LMG 28257(T)).
- 出版日期2016-1
- 单位武汉大学