A new hydrogen peroxide biosensor based on gold nanoelectrode ensembles/multiwalled carbon nanotubes/chitosan film-modified electrode

作者：Yang Yunhui^*; Yang Guangming; Huang Yan; Bai Huiping; Lu Xuxiao

来源：Colloids and Surfaces A: Physicochemical and Engineering Aspects , 2009, 340(1-3): 50-55.

DOI：10.1016/j.colsurfa.2009.02.038

• Multiwalled carbon nanotubes
• Au nanoelectrode ensembles
• Direct electrochemistry
• Electrodeposition
• Hydrogen peroxide
• Biosensor

Gold nanoelectrode ensembles were produced by electrodeposition using multiwalled carbon nanotubes (MWNTs) as template. A new third generation amperometric biosensor for hydrogen peroxide was developed based on adsorption of horseradish peroxidase (HRP) at the glassy carbon (GC) electrode modified with Au nanoelectrode ensembles/multiwalled carbon nanotubes/chitosan film. The resulting HRP biosensor offered an excellent detection for hydrogen peroxide at -0.11 V with a linear response range of 2.08 x 10(-7) to 7.6 x 10(-3) M with a correlation coefficient of 0.998, and response time < 5 s. The detection limit was 1.02 x 10(-7) M at 3 sigma. The biosensor displays rapid response, expanded linear response range, and excellent repeatability. The simple and fast fabrication of the sensor makes it superior to other techniques.

• 出版日期2009-5-15
• 单位云南师范大学; 昆明医科大学